These data were obtained because we were searching for antibiotic-producing soil bacteria. We need new antibiotics because of the prevalence of resistant bacteria – we are a part of the Small World Initiative. We took soil and did serial dilutions; we did Gram stains on four isolates, we identified an organism through a spread patch (since we skipped that lab the methods can just say “The antibiotic-producing isolate was identified through the spread patch method.”. Then we used all our biochemical tests as well as DNA isolation, sequencing, and Geneious to identify the organism. Sometimes the biochemical data conflicts with what the sequence identified which can be covered in the discussion. The outline for the lab report is at the end of this document.
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